By Sydney Crawcour

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B) Semilogarithmic plot (first-order analysis) of the area over fluorescence induction, revealing the biphasic nature of PSII photoconversion. The relative amount of PSII s centers (23 0 /0 of the total PSII) was determined from the value of the y-axis intercept with the slower linear phase (dashed line). FIGURE 2. The flash saturation properties of PSII a and PSII s in DCMU-poisoned spinach chloroplasts. The dashed lines represent the maximum changes obtained with a single saturating flash. The preilluminating flash was administered 3 ms before the onset of the actinic illumination.

23: 87-112. Holzwarth AR, 1986a. Fluorescence lifetimes in photosynthetic systems. Photochem. Photobiol. 43: 707-725. Holzwarth AR, 1986b. Excited state kinetics of chlorophyll antenna pigments. , pp. 299-309, Springer, Berlin. Holzwarth AR, 1987. Picosecond fluorescence spectroscopy and energy transfer in photosynthetic antenna pigments. , pp. 95-157, Elsevier, Amsterdam. Holzwarth AR, Wendler J and Haehne1 W, 1985. Time-resolved picosecond fluorescence spectra of the antennna ch10rophy11s in Ch10re11a vulgaris.

1985; Graan/Ort. 1986]. Following the nomenclature of Lavergne [1982]. these centers are termed PSII-QB-nonreducing in order to distinguish them from the PQ reducing PSII centers (QB-reducing). It is possible to measure the relative concentration of PSII-QB-nonreducing centers in vivo. e •• in intact leaf discs or algal cells in the absence of herbicides [Mel is, 1985]. The method is based on the inability of these centers to transfer electrons from Qj to QB. hence. upon illumination. their photochemical activity is revealed by an initial fluorescence yield increase from Fo to the intermediate plateau Fpl [Forbush/Kok.

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An introduction to kambun by Sydney Crawcour

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